Flow cytometry can be used to measure the DNA content of abnormal cellular populations and to determine percentages of cells in various phases of the cell cycle. Stain: Propidium Iodide.
Assessment of DNA/S-Phase using flow cytometry is one of the most extensively investigated prognostic parameters in cancer biology. High S-Phase in breast cancer is associated with unfavorable prognosis.
CPT Codes:
88182(1)
Specimen Requirements:
Please Note: For specimens not listed below, please contact our Client Services Department to determine if they may be acceptable.
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Fixed Paraffin Block with Corresponding H&E:
- Tissue should be well-fixed in formalin. If alternative fixative is used, it should be noted on the requisition.
- 0.2 cm x 0.2 cm x 0.2 cm tissue.
- Store specimen at room temperature (20-23.5°C).
- Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
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Fresh Tissue Biopsy:
- Two (2) pieces 0.2 cm x 0.2 cm x 0.2 cm (8-10 pieces preferred) in transport medium. Do not use transport medium if it turns cloudy, yellow or is beyond the expiration date. Use only pink/orange color media.
- Refrigerate specimen. Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
- All specimens should be shipped to Genzyme on the day of excision. Delay in shipping may compromise cell viability and results.
Turnaround Time:
2-3 days
Client Services:
Oncology/Pathology
800-447-5816
Specimen Services/Pickup:
877-246-1226