IHC analysis of breast cancer markers provides prognostic information to help the physician determine the risk of recurrence and predict response of certain types of therapies for the breast cancer patient. Assessment of DNA/S-Phase by flow cytometry is used as a prognostic marker in breast cancer. High S-Phase is associated with high histologic grade and less favorable prognosis when compared to tumors with lower S-Phase.
Information Sheet - Breast Cancer Profiles (PDF)
ER/PR
CPT Code:
88360(2)
Breast Cancer Profiles with HER2 (FISH):
ER/PR & HER2 (FISH)
CPT Codes:
88360(2), 88368(2)
ER/PR, DNA, Ki-67 & HER2 (FISH)
CPT Codes:
88182(1), 88360(3), 88368(2)
ER/PR, Ki-67 & HER2 (FISH)
CPT Codes:
88182(1), 88360(4), 88368(2)
Breast Cancer Profiles with HER2 (IHC):
ER/PR & HER2 (IHC)
CPT Code:
88360(3)
ER/PR, Ki-67 & HER2 (IHC)
CPT Code:
88360(4)
ER/PR, Ki-67, DNA & HER2 (IHC)
CPT Codes:
88182(1), 88360(4)
ER/PR, Ki-67, DNA, P53 & HER2 (IHC)
CPT Codes:
88182(1), 88360(5)
Specimen Requirements:
Please Note: To comply with the new HER2 guidelines, only formalin-fixed tissue samples will be accepted for HER2 IHC and FISH testing. Time to tumor fixation should be as soon as possible and time of fixation should be for 6-48 hours.
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Fixed Paraffin Block with Corresponding H&E:
- Tissue should be well-fixed in formalin. If alternative fixative is used, it should be noted on the requisition.
- 0.2 cm x 0.2 cm x 0.2 cm tissue.
- Store specimen at room temperature (20-23.5° C).
- Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
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Unstained Slides:
- Send all slides within 6 weeks of cutting.
- Minimum of 4 slides per antibody.
- Precut slides from paraffin blocks in 5 micron sections.
- Mount on Poly-L-Lysine-coated or plus (+) slides.
- Air dry. Do not oven dry.
- Store specimen at room temperature (20-23.5° C).
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Stained Slides:
- Only H&E or Papanicolaou-stained slides are acceptable.
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Body Fluids:
- Process fluids into formalin-fixed, paraffin-embedded cell blocks or cytospin preparation.
Turnaround Time:
2-3 days
Please Note: If any other combinations are requested, each test will be billed separately. All immunohistochemistry-stained slides, paraffin block(s) and client H&E(s) will be returned upon completion.
Client Services:
Oncology/Pathology
800-447-5816
Specimen Services/Pickup:
877-246-1226