Detects instability of microsatellite loci associated with a loss or suppression of DNA mismatch repair system. Detects instability in 5 mononucleotide markers.
CPT Codes:
83891(2), 83900(1), 83901(8), 83907(1), 83909(2), 83912-26(1), 88381(1)
Specimen Requirements:
Please Note: This assay requires submission of normal tissue (normal colonic mucosa or normal tissue from other sites of the same patient or patient’s peripheral blood sample) for comparison with tumor.
- Peripheral Blood (for control for MSI):
- 10-20 ml in EDTA (lavender-top) tube.
- Refrigerate specimen. Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
- Fixed Paraffin Block with Corresponding H&E:
- Tissue should be well-fixed in formalin. If alternative fixative is used, it should be noted on the requisition. Average tissue size 5 mm.
- 0.2 cm x 0.2 cm x 0.2 cm tissue.
- Store specimen at room temperature (20-23.5° C).
- Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
- Precut 5-10 Slides of Microsections from Each Paraffin Block Submitted
- Send all slides within 6 weeks of cutting.
- Precut slides from paraffin blocks in 7 micron sections.
- Mount on uncoated slides.
- Air dry. Do not oven dry.
- Store specimen at room temperature (20-23.5° C).
Turnaround Time:
6-7 days
Client Services:
Oncology/Pathology
800-447-5816
Specimen Services/Pickup:
877-246-1226